Chromatographic determination of Evans blue in plasma and serum

Chromatographic determination of Evans blue in plasma and serum.

The methods at present available for the determination of the dye, Evans blue, in plasma have certain disadvantages. Direct colorimetry of dyed plasma is inapplicable where the plasma is haemolyzed, turbid or lipaemic. The butanol extraction procedure of Harington, Pochin & Squire (1940) is troublesome and time-consuming where many analyses must be carried out; its accuracy is lower than those ...

Liquid-chromatographic determination of zomepirac in serum and plasma.

We describe a determination of zomepirac concentration in plasma and serum by reversed-phase "high-performance" liquid chromatography. The assay requires 1.0 mL of sample and involves diethyl ether extraction of zomepirac from an acidified sample, followed by concentration and injection into a liquid chromatograph. The column effluent is monitored at 330 nm. Retention times of zomepirac and the...

Liquid-chromatographic determination of serotonin in serum and plasma.

Using high-performance liquid chromatography with electrochemical detection, we determined serotonin in plasma from parkinsonian patients being treated with L-3,4-dihydroxyphenylalanine or N-(DL-seryl)-N'(2,3,4-trihydroxybenzyl)hydrochloride plus L-3,4-dihydroxyphenylalanine ("Sinemet") and in serum from a blood bank, from "normal" persons, and a pooled specimen from a hospital clinical laborat...

The estimation of Evans blue in plasma.

A comparison of direct and extraction methods for the determination of T-1824 (Evans blue) in plasma and serum.

The dye T-1824 (Evans Blue) has gained wide acceptance for determining plasma volume. Recently there has been a renewed interest in dye circulation studies for the measurement of cardiac output, central volume, cardiac shunts, and valvular regurgitation. The method of estimation most commonly used is spectrophotometric analysis of the dye-tinged plasma (or serum) compared with standard dye solu...